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Recombinant protein expression: From beginner

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Microbial systems have revolutionized biochemistry due to their ability to produce recombinant proteins. We are fast approaching the end of the days when a given protein could be purified with kilograms of plant or animal tissue or large amounts of biological fluid. In any new research project requiring purified proteins, researchers immediately consider recombinant forms of those proteins. It allows the biochemical characterization, use in industrial processes, and development of commercial products when a recombinant protein can be expressed and purified in large quantities.

In order to express recombinant proteins in bacteria, DNA fragments (open reading frames, ORFs) need to be inserted into an expression vector, routinely a plasmid, and this vector needs to be transferred into bacterial cells (transformation). The cells are then cultured and induced to produce recombinant proteins.

This online course will teach you how to use the microbial expression system (Bacteria: pET) for your recombinant protein expression. Learn about the bacterial manipulation to improve protein production.

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Vaccine design

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In silico vaccine design, information and tools play a special but complementary role. In silico vaccine design may be a subset of emerging immunoinformatics. Immunoinformatics may be a link between immunology and bioinformatics. this system may be very effective in vaccine design, predicting system activity, testing for allergens, and drug design. The database is quite crucial in this strategy. Various bioinformatics software that can check information for vaccine design are known as in silico tools. Naturally, when the Corona virus appeared, you grasped the necessity of drug and vaccine design. Scientists in this discipline are hosting exhibits and scientific gatherings in most nations these days in order to develop effective chemical compounds to treat various infections.